Accumulation of vasicine and vasicinone in

Evid Based Complement Alternat Med.

Accumulation of vasicine and vasicinone in

Review History Abstract Xerophytes play an active role in preventing soil denudation and desertification in arid and semi-arid areas. Zygophyllaceae familya seasonally growing, poisonous and drought-tolerant plant, is widely distributed in the Xinjiang Uygur Autonomous Region and used as a traditional herbal medicine as well as, in winter, a fodder source.

Previous research has focused on the pharmacological activity of isolated compounds and stress responses to growth environments. However, the metabolic profile of P.

Here, we collected plant samples in May, August, October and December. We determined the metabolic composition of methanol extracts using NMR spectroscopy, and comparisons of four growth stages were accomplished by applying statistical analysis.

The results showed that vasicine, choline and sucrose were significantly elevated in samples harvested in May. Significantly higher amounts of betaine, lysine, 4-hydroxyisoleucine and proline were found in samples collected in August than in samples collected in other months, and the concentrations of phosphorylcholine, glucose, acetic acid and vasicinone were highest in December.

The relationships between differential biomarkers and plant physiological states affected by diverse growth environmental factors were discussed.

Our result deepened the understanding of metabolic mechanisms in plant development and confirmed the advantage of using NMR-based metabolomic treatments in quality evaluation when Accumulation of vasicine and vasicinone in.

Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine and vasicinone in callus culture was detected and confirmed by thin layer chromatography. The Rf values of vasicine and vasicinone were found as and Keywords: Adhatoda vasica, Callus . Vasicine (peganine) is a quinazoline alkaloid. It is found in Justicia adhatoda, after which the it is named. It is additionally found in Peganum harmala. Vasicine has been compared to theophylline both in vitro and in vivo. It has also been studied in combination with the related alkaloid regardbouddhiste.comlity in acetone, alcohol, chloroform: Soluble. Information, products and services for using livestock to control weeds, reduce fire danger and improve wildlife habitat.

Introduction Peganum harmala L. The most common habitats of this plant are semi-arid rangeland, steppe areas and sandy soils [ 1 ].

This vegetation is widely distributed in the Xinjiang Uygur Autonomous Region in the northwest area of China, which covers approximately 2 million hectares [ 2 ]. As a drought-resistant and salt-tolerant species [ 1 ], P.

However, in dry years, domesticated animals, especially young individuals, are susceptible to poisoning by P. In addition, the aboveground part of P. A series of studies showed that P. In consideration of the potential medicinal and commercial values of this wild pharmaceutical plant, studies on the target metabolites of P.

Vasicinone is a quinazoline alkaloid. It shows bronchodilatory activity in vitro but bronchoconstrictory activity in vivo. Vasicinone was shown to have an antianaphylactic action. It has been found within Peganum harmala. Vasicinone has also been studied in combination with the related alkaloid regardbouddhiste.com mass: g·mol−1. International Journal of Research in Pharmacy and Science accumulation of quinazolines namely vasicinone, vasicine and vasicinol β. Vasicine (peganine) is a quinazoline alkaloid. It is found in Justicia adhatoda, after which the it is named. It is additionally found in Peganum harmala. Vasicine has been compared to theophylline both in vitro and in vivo. It has also been studied in combination with the related alkaloid regardbouddhiste.comlity in acetone, alcohol, chloroform: Soluble.

Harmine increases human pancreatic beta cell replication by interaction with dual specificity tyrosine-regulated kinase-1a [ 13 ]. The antiproliferative activity of alkaloids vasicinone, harmine, and harmalacidine isolated from P. Additionally, the extract of P.

Meanwhile, the response and tolerance of P. Since plants contain multiple chemical constituents, including alkaloids, steroids, flavonoids, anthraquinones, amino acids and inorganic elements [ 19 ] and the chemical composition of P.

Vasicine - Wikipedia

Ecological metabolomics are an effective and promising approach for elucidating ecological topics in multiple areas, including stress response, life-history variation, species lifestyle, population structure, nutrient cycles and trophic relationships [ 20 ].

Metabolic pathways and other biological processes are regulated by plant development as well as other influential factors from the environment, resulting in fluctuations at the metabolic level [ 2122 ].

As a burgeoning methodology, metabolomics addresses the concomitant linkage between latent metabolic biomarkers and causative physiological mechanisms through a multivariate analysis of complex datasets [ 2324 ]. Nuclear magnetic resonance NMRwhich can simultaneously measure all proton-containing metabolites, is one of the most commonly used methods for metabolite detection in plant science [ 25 ].

NMR techniques combined with chemometric techniques have successfully revealed metabolic variations accompanying animal and plant growth [ 26 ]. Currently, although this xerophyte and halophyte plant has shown high medicinal and commercial value, there is no metabolomics-based systematic investigation of the metabolome of P.

In this study, dynamic metabolic changes during the growth of wild P. We depicted the metabolic profiles of P. The result showed that a metabolomic approach is a powerful tool to accurately discriminate growth stages and that several key biomarkers can be easily quantified to aid in depicting molecular features.

This investigation provides data that can be used in the quality control of this medically and commercially significant wild plant and serve as a basis for future P. Material and methods 2. This region is located at the northern slope of the Tianshan Mountains and the southern edge of Junggar basin, with an altitude of — m.

Under the typical continental-arid climate, the annual natural precipitation of Changji was The mean annual air temperature is 7. The data showed that this area received abundant sunshine, with an annual duration reaching h.

Climate data of four harvest months i. The details of plot design for sampling are described in electronic supplementary material, File S1. A total of 24 samples were air-dried, ground to powder and then mixed thoroughly. Due to the loss of moisture in plant tissue readily occurring in a short time, metabolite decomposition caused by enzyme-mediated reactions is well inhibited.

All coarse particles in sieving step were ground again until all sample powder passed through the sieve, preventing bias.

Introduction

Polar metabolite extraction and sample preparation for NMR First, 2. The mixture was vortexed Thermomixer comfort, Eppendorf, Hamburg, Germany for 30 s at room temperature and then sonicated ultrasonic cleaners, Kunshan Ultrasonic instruments Co.Antimicrobial, antioxidant, and cytotoxic properties of vasicine such as Vasicine, vasicinone, Accumulation of vasicine and vasicinone in tissue.

Roja G, Vikrant BH, Sandur SK, Sharma A, Pushpa KK () Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. accumulation of ROS has been postulated to be implicated in the aging process (Beckman and vasicine, vasicinone etc have been isolated from J.

Accumulation of vasicine and vasicinone in

Roja G, Vikrant BH, Sandur SK, Sharma A, Pushpa KK () Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts.

The vasicine and vasicinone contents in these extracts were % and % of dry weight and the water extracts of the selected elite parent plant contained 3–4% dry weight of vasicine.

Vasicinone, an autooxidation product of vasicine, has been reported to cause bronchodilatory effects, both in vitro and in vivo (Shinawie, ). Bhide, Naik, and Ghooi () reported the bronchodilatory, cardiac-stimulating and anti .

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